Regulatory
Part:BBa_K2136013
Designed by: Livia Seno Ferreira Camargo Group: iGEM16_USP_UNIFESP-Brazil (2016-10-13)
hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas
AR1-Rbcs2
Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes. This part also includes a 5'-UTR derived from the rbcS2 gene.
Improvement
NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: BBa_K2516001
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 264
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//promoter
None |